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MCF7 dCas9-KRAB ATCC
WebOct 5, 2024 · Indicated KRAB-dCas9 fusions were recruited to the CD81 promoter (left) in A375 and HEK293T cells or to the SV40-EGFP reporter in HEK293T cells (right) and EGFP fluorescence was measured by flow ... WebCharacteristics: Using CRISPR/Cas9 a dCas9-TagBFP-KRAB construct is expressed from a CAGGS promoter edited into the 2nd intron of the CLYBL safe harbor. Transfected … collapsing radiator hose
AICS-0090-391
WebJun 18, 2024 · On day 1, check GFP and BFP signals under epifluorescence microscope (usually >90% efficiencies). Replaced with fresh NPC medium containing Dox, hygro, and G418 (optionally with puromycin if concerned with the loss of dCas9-KRAB transgene in NPCs) (see troubleshooting 5). c. On day 3, a large number of cells may die due to … WebThis invention includes human induced pluripotent stem cell (iPSC) lines that harbor a single copy dCas9-BFP-KRAB at the CLYBL safe harbor locus (mediating CRISPR inhibition of human gene expression) and/or a single copy of dox-inducible NGN2 at the AAVS1 locus (enabling the differentiation of the iPSCs into neurons). WebThe plasmid contains homology arms to direct insertion of a cassette into the CLYBL safe-harbor locus in the human genome. The cassette expresses CRISPRi machinery using a CAG promoter. ... The CRISPRi machinery consists of double degron-tagged dCas9-BFP-KRAB. Addition of the small molecule trimethoprim to cell culture media stabilizes the ... dropship modern decor