WebTriton™ X-100 solution BioUltra, for molecular biology, ~10% in H2O; CAS Number: 9036-19-5; Synonyms: Triton™ X-100,4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol solution,t-Octylphenoxypolyethoxyethanol,Polyethylene glycol tert-octylphenyl ether; Linear Formula: t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10; find Sigma-Aldrich-93443 MSDS, … WebSep 16, 2024 · The lysis solution is prepared with 9% (v/v) Triton X-100, whereas to prepare the stop solution, 50% DMF and 20% SDS at pH 4.7 are used. Alternatively, 1 N HCl can also be used to stop the reaction; however, DMF–SDS solution is advantageous in cell culture medium containing Phenol Red, as it neutralizes the background absorption.
Triton X-100 - an overview ScienceDirect Topics
WebProtocol A: Protocol off immunofluorescence colouring of cells in combination with propidium iodide staining of cells for cell cycle analysis. Reagents. PBS 70% ethanol 2% (w/v) paraformaldehyde inbound PBS 0.1% saponin (w/v) Propidium ionized (PI) Nucleic acid staining solution (1x PBS, 100 ug/mL RNAse A) Methods. Prepare cells appropriate. Web2. Wash the slides 2x5 min in TBS plus 0.025% Triton X-100 with gentle agitation. 0.025% Triton X-100 in the TBS reduces surface tension, allowing reagents to cover the whole tissue section with ease. It is also believed to dissolve Fc receptors and reduce non-specific binding. We recommend TBS over PBS to get a cleaner background. 3. rochesters waukesha wi
Propidium Iodide Triton X 100 Staining Solution Bioz
WebFeb 26, 2008 · Prepare propidium iodide (PI)/Triton staining solution with RNAse A. Recipe: 10 ml of 0.1% (v/v) Triton X-100 (Sigma) of PBS (Triton is a viscous liquid), 2 mg DNAse-free RNAse A (Sigma), 200 microliters of 1 mg/ml PI (Molecular Probes). Prepare fresh! 2. Warm tubes to 37°C for 5-10 minutes (otherwise ice crystals can tear apart your … WebMay 26, 2024 · For permeabilization, cells were treated with 0.1% Triton X-100 (v/v) for 5 min prior to blocking. After one remaining wash, the cells were incubated overnight at 4 °C with the rabbit monoclonal NOX4 (47-6) antibody at 2 … WebPI staining solution (with Triton X-100 and RNAase) To 10 ml of 0.1% (v/v) Triton X-100 (Sigma) in PBS add 2 mg DNase-free Rnase A (Sigma) and 200uL of 1 mg/ml PI ** … rochesteryc.com