WebbMitochondrial ROS (mtROS) was detected by MitoSOX™ Red mitochondrial superoxide indicator (M36008, Thermo Fisher Scientific, United States). According to the manufacture’s instruction, cells were loaded by adding the 5 μM MitoSOX™ reagent working solution prepared and then incubated for 20 min at 37°C, protected from light. Webb9 mars 2015 · The cells were then washed with PBS and analyzed on a Thermo Fisher Cellomics ArrayScan® VTI. A 1 0 50 100 150 200 250 300 350 400 Control Menadione Control + NAC Menadione + NAC ***a ***b B C 0 50 100 150 200 250 300 350 ***b 350 0 50 100 150 200 250 300 ... M36008 MitoSOX® Red mitochondrial superoxide indicator …

Ratio of CD4+ and CD8+ T cells of live CD3+ cells 12 days

http://www.maokangbio.cn/Products-18555260.html WebbFinally, gels were imaged with Gel Doc XR+System (Thermo Fisher Scientific) and analyzed with Image J (National Institutes of ... and incubated with 1x PBS containing 5 μM MitoSOX (Invitrogen, Waltham, MA, USA, #M36008) for 40 min at 37 °C in a dry oven. The specimens were washed with 1x PBS. The slides containing ovarian tissues were ... ppum webmail

(PDF) ALDH2 dysfunction accelerates ESCC pathogenesis

http://www.labgic.online/ Webb25 okt. 2016 · The M2 isoform of pyruvate kinase, muscle (PKM2), catalyses the final and also a rate-limiting reaction in the glycolytic pathway. PKM2 is present in few types of proliferating normal cells, but is... Webb1 jan. 2024 · This protocol suggests a quick way of evaluating mitochondrial fitness using flow cytometry. We propose using the probes MitoTraker Green and MitoTracker Red/ MitoSOX at the same time. This way, it is possible to evaluate different parameters of mitochondrial biology in living cells. Flow cytometry is a highly used tool by … ppum ward charges